Visualize binding curves under different conditions
This simulator will draw the binding curve over a selected concentration range of titrated species given the selected affinity (KD) and probe concentration. The curve is scaled to hydrodynamic radius (Rh), transitioning from purely unbound probe to complex. The simulator can be useful in modelling a reaction of interest and for developing an understanding of how binding curves change as input parameters are adjusted.The equation for the curve shown is:
where Rh is the expected Rh value at the indicated probe concentration, Rh,free is the Rh of the free, unbound labeled probe, Rh,complex is the Rh of the complex, and [A] and [B] are the total concentrations of labeled probe and unlabeled target, respectively.