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This guide provides guidance for users who want to characterize the stoichiometry as well as the affinity of an interaction. The guide provides a generic protocol to be used on the Fluidity One-M
that can be easily adapted for any protein and includ...
This guide provides guidance for users who want to characterize interactions with membrane proteins without purification or use of detergents. The guide provides a generic protocol to be used on the Fluidity One-M that can be easily adapted for any m...
We built our Fluidity One-M instrument with your challenges in mind. Analysis in-solution on our single-use microfluidic channels that are optimized for running crude membrane preparations eliminates artefacts. The smart assistant – Fluidity Insig...
A new type of quantitative serum-antibody profiling: When studying the immune response to a drug, a vaccine, or an antigen, established serological technologies, such as ELISA, only provide semi-quantitative outputs such as titer.
Protein aggregates in
neurodegenerative
diseases: Protein aggregates are clusters of misfolded proteins in the form of oligomers, tangles, plaques, and fibrils. A wide range of neurodegenerative diseases, including Alzheimer’s disease, Parkinson’...
The making of therapeutic antibodies: Antibodies represent one of the most prevalent therapeutic approaches, with a wide range of variations available to optimize function across oncology, neurology, and immunology. The successful transition from de...
This guide provides a generic protocol to profile antibodies in plasma or serum on the Fluidity One-M system. This can be easily adapted for any protein and includes helpful tips and tricks to successfully analyze protein interactions on the Fluidity...
The guide provides a generic protocol for labeling, which yields products suitable for use on the Fluidity One-M platform. This can be easily adapted for any protein.
This guide is for users who want to
characterize protein interactions by generating affinity binding curves
with their proteins of choice. The guide provides a generic protocol to be used on Fluidity One-M systems that can be easily adapted for any...
Quantify binding affinity, size,
concentration and stoichiometry
in solution, all at once. Proteins play criticalroles in the human body, maintaining
and regulating the structure and function of tissues and
organs.
- Videos
Understand what binding curves are, how to create them and tips to avoid pitfalls.
Take a deep dive into binding affinity and the dissociation constant KD.
A new class of in-solution protein interaction measurement. MDS gives one simple and direct measurement – protein molecular size. When binding occurs, changes in protein size are monitored and used to determine binding affinity and calibration-free...
- All
- Autoimmune disease
- Biochemistry
- Biophysics
- Biotherapeutic Development
- Cardiology
- Immunology
- Infectious disease
- Neuroscience
- Oncology
Spagni, G. et al. "Serological Markers of Clinical Improvement in MuSK Myasthenia Gravis" Neurology Neuroimmunology & Neuroinflammation 11 (2024) e200313
Bocharov, Eduard et al. "All-d-Enantiomeric Peptide D3 Designed for Alzheimer’s Disease Treatment Dynamically Interacts with Membrane-Bound Amyloid-β Precursors" Journal of Medicinal Chemistry 64 (2021):16464-16479
Baumann, Kevin N. et al. "A Kinetic Map of the Influence of Biomimetic Lipid Membrane Models on Aβ42 Aggregation" ACS Chemical Neuroscience 14 (2023): 323-329
Bernardim, B. et al. "Cathepsin B Processing Is Required for the In Vivo Efficacy of Albumin–Drug Conjugates" Bioconjugate Chemistry 35 (2024): 132-139
Challa, Pavan K. et al. "Real-Time Intrinsic Fluorescence Visualization and Sizing of Proteins and Protein Complexes in Microfluidic Devices" Analytical Chemistry 90 (2018) 3849-3855
Chia, S. "A Relationship between the Structures and Neurotoxic Effects of Aβ Oligomers Stabilized by Different Metal Ions" ACS Chemical Neuroscience 15 (2024): 1125-1134
Lázaro-Gorines, R. "Dendritic Cell-Mediated Cross-Priming by a Bispecific Neutralizing Antibody Boosts Cytotoxic T Cell Responses and Protects Mice against SARS-CoV-2" Advanced Science 10 (2023): 2304818
O'Mahoney, Cara et al. "Microfluidic Diffusional Sizing (MDS) Measurements of Secretory Neutralizing Antibody Affinity Against SARS-CoV-2" Annals of Biomedical Engineering 52 (2024): 1653-1664
Kelly, Colleen M., Jody L. Martin, and Michael J. Previs. "Myosin folding boosts solubility in cardiac muscle sarcomeres." JCI insight 9.8 (2024).
- All
- Tools
This simulator will draw the binding curve over a selected concentration range of titrated species given the selected affinity (KD) and probe concentration. The curve is scaled to hydrodynamic radius (Rh), transitioning from purely unbound probe to c...
An online calculator of hydrodynamic radius from molecular weight. Determine the theoretical hydrodynamic radius of your protein or protein complex before running it on the Fluidity One-M.
Online calculator of hydrodynamic radius from structural models using HullRad. Helps interpret the microfluidic diffusional sizing measurements obtained on Fluidic Sciences instruments.
Check out our Labeling Calculator, a tool that helps with volume and concentration calculations when labeling proteins required for MDS assays. This calculator is designed to work with our NHS ester labeling guide which provides detailed guidance on ...