Fluidity One-M

Discover faster – without getting lost

Measuring molecular interactions can be tough. But we’ve got your back.

Start simple in less than 1 day

Your time is precious, it should be spent on exploring the insights, not getting lost among complex protocols. Get started easily and confidently with a simple mix-and-measure workflow. No training is required.

Get data delivered in 25 minutes

Fluidity One-M is built to help you move faster to insights. It takes 25 minutes to generate 24 data points, providing a full binding curve and KD value. Go grab some lunch and come back to see your publication-ready results.

Preparation

10 minutes

Transfer

3 minutes

Measurement

25 minutes

Analysis

2 minutes

See a full picture without assumptions

Affinity alone can’t tell a complete story about how proteins truly interact. Fluidity One-M provides multiple metrics to help you fully assess the intricacies of protein interactions.

Molecular size

Fluidity One-M measures molecular size (hydrodynamic radius) of a sample by evaluating its diffusion in a microfluidic chamber.

Binding affinity

The size of the protein is measured in free form and against a titration of binding partner, allowing construction of a binding curve and calculation of binding affinity (KD).

Calibration-free concentration

The concentration of active binding sites can be determined by measuring binding at different concentrations of the labelled protein.

Stoichiometry

Concentration data can also be used to determine binding stoichiometry (number of binding sites per molecule).

 

​Measure in solution, break the constraints

Swiftly overcome the common challenges of traditional protein characterization methods and explore the behaviours of any proteins in their native environments.

No immobilization

With Fluidity One-M, you can measure interactions directly in solution without having to immobilize your proteins on a surface.

No purification

No purification is needed. Fluidity One-M can work with crude samples, saving you preparation time and simplifying the workflow.

No background constraints

Confidently tackle complex backgrounds such as serum, plasma or cell lysate that are difficult for traditional methods.

No target constraints

Analyze any targets from traditional to challenging ones, such as protein aggregates, membrane proteins, disordered proteins, and PROTACs.

What our users say

“MDS allows simultaneously determining both concentration and affinity of donor-specific antibodies under native conditions, directly in clinical samples, which is highly valuable.

MDS concentration/affinity tests have the potential to replace expensive antibody titre-determinations.

We particularly appreciate the high degree of standardisation of this approach, making it well suited for use in clinical laboratories.”

Dr Gideon Hoenger
University Hospital Basel

“We initially developed the MDS technology in our lab and have now switched to using the Fluidity One M instrument for all of our MDS and protein interaction work because it’s very robust and convenient.”

Prof. Tuomas Knowles
University of Cambridge

“MDS allowed us to obtain unique information that helped us elucidate, for the first time, the way antibody therapeutics bind to different forms of their targets.”

Prof. Sara Linse
Lund University

We pay attention to detail, so you can pay attention to discoveries.

No cross-contamination

Avoid cross-contamination biases while saving cost with our single-use plate-based consumables

Low maintenance

Avoid clogging, priming, or flushing because no fluidics are in the instrument

Fear of getting lost? Not with us

You are not alone on this journey. Fluidity One-M is supported by Fluidity Insight, an analytics platform and co-pilot to keep your experiment design on the right track.

Fluidity Insight supports you with machine learning guided assay development, letting you know which datapoints you can add to your existing dataset to best improve the fit quality of your experiment.

Start small. Gain big.

And move fast.

4 µL

Sample volume per data point, enabling KD determination with as little as 50 µL of sample

1-20 nm

Range for size measurement, covering peptides to multiprotein complexes and fibrillar aggregates

24 data points

Number of data points generated in one single experiment

from pM to µM

Range for affinity measurement, covering high and low affinity systems

See how Fluidity One-M accelerates real-world projects

    • Highlights
    • Publications

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    Looking for more information?

    Find out more about the instrument and our consumables, and explore a collection of articles, documents, videos and other resources to learn more about our technology and products, or contact us for more information.

    Consumables

    Fluidity One-M Chip-plates

    Two boxes each containing eight chip-plates

    These chip-plates are for use with the Fluidity One-M instrument to characterize protein interactions, including the determination of affinity (KD), size, concentration, and stoichiometry.

    News & Blogs

    Stay updated with the latest news, knowledge and trends in protein interaction research.

    Resources

    Access a library of resources about our technology and products, covering educational videos, user guides, application notes, publications and many more.

    Fluidic Sciences Ltd