Measure in solution and accelerate progress with MDS
Start quickly, start small
- No immobilization or purification required – just mix and measure
- Works for low sample volume
Grab a full picture in 25 mins
- 25 minutes to data
- Size, binding affinity (KD), stoichiometry and concentration all at once
Break the barriers
- No surface constraints
- Works for any targets and backgrounds
See what really happens without bias
- No calibration and no prior biophysical knowledge required
- Quantify binding events in their native state and native environments
How it works
Microfluidic Diffusional Sizing (MDS) measures molecular size (hydrodynamic radius) of a sample by evaluating its diffusion in a microfluidic chamber.
A fluorescently labelled protein enters the microfluidic diffusion chamber together with an auxiliary fluid, in two parallel laminar streams.
The rate of diffusion of protein from one stream to the other depends on the protein’s molecular size.
The rate of diffusion and hydrodynamic radius (Rh) of the protein can be calculated based on the ratio of fluorescence signal detected in the two streams at the end of the chamber.
Changes in size reveal protein interactions
The size of the protein is measured against a titration of binding partner, allowing construction of a binding curve and calculation of binding affinity (KD).
See beyond affinity
The concentration of active binding sites can be determined by measuring binding at different concentrations of the labelled protein. This provides a calibration-free measurement of concentration in complex samples such as biofluids or crude membrane preparations.
Concentration data can also be used to determine binding stoichiometry (number of binding sites per molecule) with the support of the smart assistant Fluidity Insight.
Reach new possibilities
Confidently work with challenging targets and backgrounds that are difficult for traditional methods, because MDS requires no immobilization and purification.
From traditional to challenging targets
- Globular proteins
- DNA
- Antibodies
- Bispecific antibodies
- Amyloid fibrils
- Polyclonal antibodies
- Disordered proteins
- PROTACs
- Membrane proteins
In purified or complex backgrounds
- Buffers
- Purified samples
- Diluted serum and plasma
- Fermentation broth
- Saliva
- Serum and plasma
- Cell lysate
- Tissue homogenate
- Clinical samples
Get ahead in every stage of drug development
MDS enables a wide range of applications throughout different stages of drug development process.
Basic Research
- Measure binding and activity of your most challenging targets
- Unlock competition experiments and work with ternary complexes
Drug discovery
- Validate and characterize hits without having to purify
- Optimize leads and understand their mechanism of action
Translational Research
- Obtain concentration and affinity of serum antibodies and overcome limitations imposed by ELISA titers
- Evaluate immune responses directly in patient samples
Technology comparison
- Non-specific binding
- Misfolding
- Avidity effects
- Direct use of disease-relevant proteins
- Native structure
- No avidity
- Non-native environments
- Time-consuming
- Native environments possible
- Shorter time to result
- No in-built assay control
- Ambiguous results
- Expert know-how required
- In-built assay control
- Highly reproducible and easy to interpret results
- Easy for anyone to run
- Lower number of replicates possible
- Less material available for complimentary measurements
- More replicates
- More material available for orthogonal characterization
- Extensive system preparation and lengthy assay development
- Get first data in 25 minutes
- Focus on characterizing interactions without learning more about the sample
- Achieve measurements of binding affinity (KD), hydrodynamic radius (Rh) of free probe and protein complex, concentration and stoichiometry
- Dependent on a priori assumption or on empirical calibration
- No prior knowledge required. Determine the concentration of active binding sites by measuring binding at different concentrations of the labelled protein
Peer-reviewed publications
- Highlights
- Publications
No data found.
See MDS in action
APPLICATIONS
Explore how it supports different research goals
OUR PRODUCT
Fluidity One-M
Learn how you can get MDS up and running for your project with the Fluidity One-M system.
